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| Registros recuperados: 11 | |
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| Marthe,Jefferson de Brito; Pompolo,Silvia das Graças; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia. |
| Four colonies of the stingless bee Partamona cupira (Hymenoptera: Apidae) were cytogenetically analyzed using conventional staining and the fluorochromes CMA3 e DAPI. The females have 2n = 34 chromosomes (2K=32<img border=0 src="../../../../../../../img/revistas/gmb/2010nahead/ah06a04CarM.gif" align=absmiddle>+2<img border=0 src="../../../../../../../img/revistas/gmb/2010nahead/ah06a04CarA.gif" align=absmiddle>). Some females, however, presented an additional large B acrocentric chromosome, to a total of 2n = 35. Chromosome B and the chromosomal pairs 2, 9 and 10 showed CMA3+ bands, indicating an excess of CG base-pairs. A clear association was verified between the P. helleri B chromosome SCAR marker and the presence of a B chromosome in P.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: B chromosome; SCAR; Stingless bees. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200009 |
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| Reyes-Montes,María del Rocío; Frías-De-León,María Guadalupe; Victoriano-Pastelín,Isai; Acosta-Altamirano,Gustavo; Duarte-Escalante,Esperanza. |
| ABSTRACT At present, there is no standardized marker that is routinely used in clinical laboratories to diagnose coccidioidomycosis. Thus, the goals of this study were to obtain a sequence characterized amplified region (SCAR) marker for the identification of Coccidioides spp., evaluate its specificity and sensitivity in fungal DNA-spiked blood and sputum samples, and compare it with previously described molecular markers. Specific amplified fragment length polymorphism (AFLP) amplicons for Coccidioides immitis and Coccidioides posadasii were cloned into the vector pGEM® -T Easy vector and sequenced to develop a SCAR marker. Oligonucleotides were designed to identify Coccidioides spp. by polymerase chain reaction (PCR), and the specificity and sensitivity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; SCAR; Coccidioidomycosis; Coccidioides spp.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500322 |
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| Sandhu,S.S.; Colombo,Carlos; Bastos,Cândido R.; Siqueira,Walter J.. |
| Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rice; SCAR; Arbitrary primers; Pyricularia oryzae. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400011 |
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| Oliveira,Eder Jorge de; Dantas,Jorge Luiz Loyola; Castellen,Milene da Silva; Lima,Diego Souza de; Barbosa,Helder de Souza; Motta,Tiago Borges Nunes. |
| O objetivo deste trabalho foi validar marcadores moleculares, previamente identificados como ligados ao sexo do mamoeiro, para utilização na seleção indireta em genótipos comerciais. Foram analisadas duas variedades do grupo Solo e dois híbridos do grupo Formosa, com utilização de 20 plantas por genótipo, quatro marcadores do tipo SCAR (Sequence Characterized Amplified Region) e um RAPD (Random Amplified Polymorphic DNA). O RAPD BC210 permitiu a identificação de todas as plantas femininas e hermafroditas, o que revela grande potencial para ser usado na seleção assistida em alguns dos genótipos mais cultivados no Brasil. Os marcadores do tipo SCAR não permitiram a identificação correta do sexo dos genótipos, pois detectou-se a presença de falso-positivos e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; SCAR; RAPD; Identificação sexual. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001200012 |
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| Martins Filho,Sebastião; Sediyama,Carlos Sigueyuki; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de. |
| The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; RAPD; SCAR; Soybean; Cercospora sojina; Frogeye leaf spot. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012 |
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| Parrella,Nádia Nardely Lacerda Durães; Santos,João Bosco dos; Parrella,Rafael Augusto da Costa. |
| Na obtenção de cultivares de feijão com resistência à antracnose, outros atributos agronômicos também devem ser considerados para atender a preferência do consumidor e do produtor. Neste trabalho, objetivou-se identificar famílias de feijão que reúnam, além da resistência à antracnose, alta produtividade, grãos tipo Carioca e porte ereto. Foram cruzados os genitores CNFC 10706, B1 portador do alelo de resistência à antracnose Co-4 e H147 portador do alelo de resistência Co-5. Os três possuem grãos semelhantes ao Carioca. Inicialmente foram avaliadas 224 famílias F2:3, derivadas dos três cruzamentos, mais a cultivar Talismã como testemunha, no inverno/primavera de 2004, em Lavras, com base no tipo de grão. Foram selecionadas 99 famílias F2:4 e avaliadas com... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Piramidação; Colletotrichum lindemuthianum; SCAR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000500022 |
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| Menezes,Carlos C.E; Vantoai,Tara; McDonald,Miller B; Sediyama,Tocio. |
| Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vinca; Seed; Variety discrimination; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100041 |
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| Santos,Carlos A.F.; Simon,Philipp W.. |
| Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Daucus carota; Evolution; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013 |
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| Silva,Paulo Roberto Da; Milach,Sandra Cristina Kothe; Sortica,Vinicius de Albuquerque; Boff,Tatiana; Brammer,Sandra Patussi; Federizzi,Luiz Carlos. |
| O objetivo deste trabalho foi validar marcadores moleculares previamente associados a genes que conferem resistência à ferrugem-da-folha, em genótipos brasileiros de trigo. Cinco marcadores STS e SCAR, identificados como associados aos alelos de resistência dos genes Lr1, Lr9, Lr10 e Lr24, foram avaliados por PCR, em 25 genótipos de trigo com conhecida presença ou ausência desses alelos. O marcador STS, associado ao alelo de resistência do gene Lr1, não foi eficiente em identificar genótipos brasileiros que possuem este alelo de resistência. Os marcadores STS e SCAR, associados a Lr9, Lr10 e Lr24, foram eficientes na identificação de plantas que possuem o alelo de resistência desses genes, e podem ser utilizados na seleção por marcadores da resistência à... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Puccinia triticina; Triticum aestivum; SCAR; STS. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000014 |
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| Registros recuperados: 11 | |
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